DNP = did not play in this round
| Region | Team 1 | Team 2 | ||
|---|---|---|---|---|
| Atlantic I | Society of Themistocles (Oviedo, FL) | 40 | Oak Hall School (Gainesville, FL) | 63 |
| Atlantic I | Holy Trinity Classical Christian School (Beaufort, SC) | DNP | St John's Episcopal Parish Day School (Tampa, FL) | 55 |
| Atlantic I | Thomas Hart Academy (Hartsville, SC) | 45 | Bye | 0 |
| Atlantic II | Bexley Middle & High School (Columbus, OH) | 70 | Bala Cynwyd Middle School Purple (Bala Cynwyd, PA) | 64 |
| Atlantic II | Black Rock Middle School Gold (Villanova, PA) | 56 | Bala Cynwyd Middle School Gold (Bala Cynwyd, PA) | 33 |
| Atlantic II | Black Rock Middle School Purple (Villanova, PA) | 47 | Liberty Common High School (Fort Collins, CO) | 74 |
| Atlantic III | Summit Country Day School (Cincinnati, OH) | 58 | Lakewood Montessori Middle School (Durham, NC) | DNP |
| Atlantic III | Robious Middle School Gold (Midlothian, VA) | 61 | Robious Middle School Purple (Midlothian, VA) | 57 |
| Atlantic III | Flint Hill School (Oakton, VA) | 74 | Bye | 0 |
| Central I | St. Mark's School of Texas Gold (Dallas, TX) | 69 | St. Mark's School of Texas Purple (Dallas, TX) | 60 |
| Central I | Fort Worth Country Day Middle (Fort Worth, TX) | 57 | Greenhill Middle School Purple (Addison, TX) | 65 |
| Central I | Greenhill Middle School Gold (Addison, TX) | 52 | Bye | 0 |
| Central II | Central Magnet School (Murfreesboro, TN) | DNP | BASIS Ahwatukee (Phoenix, AZ) | 78 |
| Central II | Saint Thomas' Episcopal Church & School Gold (Houston, TX) | 53 | Aristoi Classical Academy (Katy, TX) | 65 |
| Central II | Saint Thomas' Episcopal Church & School Purple (Houston, TX) | 61 | Bye | 0 |
| Midwest | The Stanley Clark School Purple (South Bend, IN) | 46 | Brookfield Academy Purple (Brookfield, WI) | 46 |
| Midwest | Northwest Ohio Classical Academy Purple (Toledo, OH) | 51 | The Stanley Clark School Gold (South Bend, IN) | DNP |
| Midwest | Northwest Ohio Classical Academy Gold (Toledo, OH) | 53 | Brookfield Academy Gold (Brookfield, WI) | 50 |
| Northeast | Dutchess Day School (Millbrook, NY) | 66 | Duxbury Middle/High School (Duxbury, MA) | 34 |
| Northeast | Oak Middle School (Shrewsbury, MA) | 52 | Bye | 0 |
| Northeast | Ridgewood High School Purple (Ridgewod, NJ) | 59 | Ridgewood High School Gold (Ridgewod, NJ) | 49 |
| Pacific | Harker Middle School (San Jose, CA) | 69 | 'Iolani School (Honolulu, HI) | 44 |
| Pacific | CHD Academy Gold (Concord, CA) | 64 | Mirman School Purple (Los Angeles, CA) | 68 |
| Pacific | Mirman School Gold (Los Angeles, CA) | 67 | CHD Academy Purple (Concord, CA) | 47 |