DNP = did not play in this round
| Region | Team 1 | Team 2 | ||
|---|---|---|---|---|
| Atlantic I | Gonzaga College High School (Washington, DC) | 40 | Oakton High School (Vienna, VA) | 32 |
| Atlantic I | Flint Hill School (Oakton, VA) | 38 | Thomas Jefferson HS for Science and Technology (Alexandria, VA) | 78 |
| Atlantic I | Hayfield Secondary School (Alexandria, VA) | 52 | Thomas A Edison High School (Alexandria, VA) | 40 |
| Atlantic II | Franklin Academy High School Purple (Wake Forest, NC) | 30 | Thales Academy Rolesville (Rolesville, NC) | 29 |
| Atlantic II | Covenant Day School Gold (Matthews, NC) | 36 | Plant High School (Tampa, FL) | DNP |
| Atlantic II | Covenant Day School Purple (Matthews, NC) | DNP | Franklin Academy High School Gold (Wake Forest, NC) | 35 |
| Central I | St. Mark's School of Texas (Dallas, TX) | 69 | Providence Preparatory School (Belton, TX) | 40 |
| Central I | Amarillo High School Purple (Amarillo, TX) | 46 | Trinity Valley School (Fort Worth, TX) | 44 |
| Central I | Amarillo High School Gold (Amarillo, TX) | 25 | Fort Worth Country Day (Fort Worth, TX) | 29 |
| Central II | Clements High School Gold (Sugar Land, TX) | 55 | Highlands Latin School Houston Gold (Houston, TX) | DNP |
| Central II | Klein Collins High School (Klein, TX) | 50 | Highlands Latin School Houston Purple (Houston, TX) | 35 |
| Central II | Clements High School Purple (Sugar Land, TX) | 38 | Aristoi Classical Academy (Katy, TX) | 41 |
| Midwest I | University School of Milwaukee (River Hills, WI) | 29 | Parnassus Preparatory School (Maple Grove, MN) | 24 |
| Midwest I | Brookfield East High School (Brookfield, WI) | 47 | Saint Louis Priory School (St. Louis, MO) | 27 |
| Midwest I | Brookfield Academy (Brookfield, WI) | 68 | Collinsville High School (Collinsville, IL) | 31 |
| Midwest II | St. Xavier High School (Cincinnati, OH) | 38 | Covington Classical Academy (Covington, KY) | 33 |
| Midwest II | North Central High School (Indianapolis, IN) | 35 | Northwest Ohio Classical Academy (Toledo, OH) | 36 |
| Midwest II | Homestead High School (Fort Wayne, IN) | 38 | St. Edward High School (Lakewood, OH) | 38 |
| Northeast I | Ridgewood High School (Ridgewood, NJ) | 30 | Hampden Academy Purple (Hampden, ME) | 36 |
| Northeast I | Stuyvesant High School Purple (New York, NY) | 21 | Hampden Academy Gold (Hampden, ME) | 40 |
| Northeast I | Stuyvesant High School Gold (New York, NY) | 49 | Garden City High School (Garden City, NY) | 25 |
| Northeast II | Boys' Latin of Philadelphia Charter High School (Philadelphia, PA) | 39 | IvyMind Academy (Garnet Valley, PA) | 84 |
| Northeast II | Harriton HS Purple (Bryn Mawr, PA) | 82 | Owen J. Roberts High School (Pottstown, PA) | 38 |
| Northeast II | Lower Merion High School (Ardmore, PA) | 61 | Harriton HS Gold (Bryn Mawr, PA) | 44 |
| Northeast III | State College Area High School Gold (State College, PA) | 33 | The Linsly School (Wheeling,, WV) | 37 |
| Northeast III | Winchester High School (Winchester, MA) | 49 | Bexley Middle & High School (Columbus, OH) | 27 |
| Northeast III | Beaumont School (Cleveland Heights, OH) | 32 | State College Area High School Purple (State College, PA) | 56 |
| Pacific I | 'Iolani School Gold (Honolulu, HI) | 43 | University High School Gold (Irvine, CA) | 48 |
| Pacific I | Pacifica Christian HS Santa Monica (Santa Monica, CA) | 30 | University High School Purple (Irvine, CA) | 44 |
| Pacific I | Sage Hill School (Newport Coast, CA) | 60 | 'Iolani School Purple (Honolulu, HI) | 24 |
| Pacific II | Basis Ahwatukee Purple (Phoenix, AZ) | 37 | Phoenix Country Day School Gold (Paradise Vly, AZ) | 35 |
| Pacific II | Trivium Preparatory Academy (Goodyear, AZ) | 58 | Basis Ahwatukee Gold (Phoenix, AZ) | 67 |
| Pacific II | Brophy College Preparatory (Phoenix, AZ) | 48 | Phoenix Country Day School Purple (Paradise Vly, AZ) | 34 |
| South I | George Walton Academy Gold (Monroe, GA) | DNP | Walton High School Gold (Marietta, GA) | 42 |
| South I | Milton High School (MIlton, GA) | 35 | George Walton Academy Purple (Monroe, GA) | DNP |
| South I | Walton High School Purple (Marietta, GA) | 60 | DeKalb School of the Arts (Avondale Estates, GA) | 41 |
| South II | Maclay School (Tallahassee, FL) | DNP | Alabama School of Fine Arts Purple (Birmingham, AL) | DNP |
| South II | Thomas County Schools (Thomasville, GA) | 26 | Oak Hall School (Gainesville, FL) | 67 |
| South II | Society of Themistocles (Oviedo, FL) | 44 | Alabama School of Fine Arts Gold (Birmingham, AL) | DNP |
| South III | Dreher HS (olumbia, SC) | 33 | Summit Country Day School (Cincinnati, OH) | 40 |
| South III | Memphis University School Purple (Memphis, TN) | 46 | Memphis University School Gold (Memphis, TN) | 39 |
| South III | Central Magnet School (Murfreesboro, TN) | DNP | Clarksville High School (Clarksville, TN) | 46 |
| West I | CHAO (Omaha, NE) | 32 | The Meadows School (Las Vegas, NV) | 44 |
| West I | The Harker School (San Jose, CA) | 84 | Liberty Common High School (Fort Collins, CO) | 38 |
| West I | Sage Ridge School (Reno, NV) | 32 | Bye | 0 |
| West II | Bye | 0 | The Classical Academy High School (Colorado Springs, CO) | 36 |
| West II | Anderson HS (Austin, TX) | 37 | Thomas Jefferson High School Purple (Denver, CO) | 23 |
| West II | Northside Health Careers HS (San Antonio, TX) | 63 | Thomas Jefferson High School Gold (Denver, CO) | DNP |