DNP = did not play in this round
| Region | Team 1 | Team 2 | ||
|---|---|---|---|---|
| Atlantic I | Gonzaga College High School (Washington, DC) | 37 | Flint Hill School (Oakton, VA) | 47 |
| Atlantic I | Oakton High School (Vienna, VA) | DNP | Hayfield Secondary School (Alexandria, VA) | 63 |
| Atlantic I | Thomas A Edison High School (Alexandria, VA) | 30 | Thomas Jefferson HS for Science and Technology (Alexandria, VA) | 87 |
| Atlantic II | Franklin Academy High School Purple (Wake Forest, NC) | DNP | Covenant Day School Gold (Matthews, NC) | 34 |
| Atlantic II | Thales Academy Rolesville (Rolesville, NC) | DNP | Covenant Day School Purple (Matthews, NC) | DNP |
| Atlantic II | Franklin Academy High School Gold (Wake Forest, NC) | DNP | Plant High School (Tampa, FL) | 54 |
| Central I | St. Mark's School of Texas (Dallas, TX) | 64 | Amarillo High School Purple (Amarillo, TX) | DNP |
| Central I | Providence Preparatory School (Belton, TX) | 38 | Amarillo High School Gold (Amarillo, TX) | 45 |
| Central I | Fort Worth Country Day (Fort Worth, TX) | 37 | Trinity Valley School (Fort Worth, TX) | 55 |
| Central II | Clements High School Gold (Sugar Land, TX) | 36 | Klein Collins High School (Klein, TX) | 57 |
| Central II | Highlands Latin School Houston Gold (Houston, TX) | DNP | Clements High School Purple (Sugar Land, TX) | 52 |
| Central II | Aristoi Classical Academy (Katy, TX) | 39 | Highlands Latin School Houston Purple (Houston, TX) | DNP |
| Midwest I | University School of Milwaukee (River Hills, WI) | DNP | Brookfield East High School (Brookfield, WI) | 52 |
| Midwest I | Parnassus Preparatory School (Maple Grove, MN) | 43 | Brookfield Academy (Brookfield, WI) | 78 |
| Midwest I | Collinsville High School (Collinsville, IL) | 31 | Saint Louis Priory School (St. Louis, MO) | 40 |
| Midwest II | St. Xavier High School (Cincinnati, OH) | DNP | North Central High School (Indianapolis, IN) | 22 |
| Midwest II | Covington Classical Academy (Covington, KY) | 46 | Homestead High School (Fort Wayne, IN) | 35 |
| Midwest II | St. Edward High School (Lakewood, OH) | DNP | Northwest Ohio Classical Academy (Toledo, OH) | DNP |
| Northeast I | Ridgewood High School (Ridgewood, NJ) | 39 | Stuyvesant High School Purple (New York, NY) | 58 |
| Northeast I | Hampden Academy Purple (Hampden, ME) | 41 | Stuyvesant High School Gold (New York, NY) | 55 |
| Northeast I | Garden City High School (Garden City, NY) | 41 | Hampden Academy Gold (Hampden, ME) | 46 |
| Northeast II | Boys' Latin of Philadelphia Charter High School (Philadelphia, PA) | 72 | Harriton HS Purple (Bryn Mawr, PA) | 73 |
| Northeast II | IvyMind Academy (Garnet Valley, PA) | 92 | Lower Merion High School (Ardmore, PA) | DNP |
| Northeast II | Harriton HS Gold (Bryn Mawr, PA) | 46 | Owen J. Roberts High School (Pottstown, PA) | 46 |
| Northeast III | State College Area High School Gold (State College, PA) | 36 | Winchester High School (Winchester, MA) | 53 |
| Northeast III | The Linsly School (Wheeling,, WV) | 32 | Beaumont School (Cleveland Heights, OH) | 34 |
| Northeast III | State College Area High School Purple (State College, PA) | 65 | Bexley Middle & High School (Columbus, OH) | 48 |
| Pacific I | 'Iolani School Gold (Honolulu, HI) | 50 | Pacifica Christian HS Santa Monica (Santa Monica, CA) | 33 |
| Pacific I | University High School Gold (Irvine, CA) | 49 | Sage Hill School (Newport Coast, CA) | 74 |
| Pacific I | 'Iolani School Purple (Honolulu, HI) | 42 | University High School Purple (Irvine, CA) | 51 |
| Pacific II | Basis Ahwatukee Purple (Phoenix, AZ) | 43 | Trivium Preparatory Academy (Goodyear, AZ) | 54 |
| Pacific II | Phoenix Country Day School Gold (Paradise Vly, AZ) | 46 | Brophy College Preparatory (Phoenix, AZ) | 47 |
| Pacific II | Phoenix Country Day School Purple (Paradise Vly, AZ) | 40 | Basis Ahwatukee Gold (Phoenix, AZ) | 77 |
| South I | George Walton Academy Gold (Monroe, GA) | DNP | Milton High School (MIlton, GA) | 46 |
| South I | Walton High School Gold (Marietta, GA) | 59 | Walton High School Purple (Marietta, GA) | 66 |
| South I | DeKalb School of the Arts (Avondale Estates, GA) | 34 | George Walton Academy Purple (Monroe, GA) | DNP |
| South II | Maclay School (Tallahassee, FL) | DNP | Thomas County Schools (Thomasville, GA) | 31 |
| South II | Alabama School of Fine Arts Purple (Birmingham, AL) | DNP | Society of Themistocles (Oviedo, FL) | 52 |
| South II | Alabama School of Fine Arts Gold (Birmingham, AL) | DNP | Oak Hall School (Gainesville, FL) | 46 |
| South III | Dreher HS (olumbia, SC) | 39 | Memphis University School Purple (Memphis, TN) | DNP |
| South III | Summit Country Day School (Cincinnati, OH) | DNP | Central Magnet School (Murfreesboro, TN) | 46 |
| South III | Clarksville High School (Clarksville, TN) | 43 | Memphis University School Gold (Memphis, TN) | DNP |
| West I | CHAO (Omaha, NE) | DNP | The Harker School (San Jose, CA) | 78 |
| West I | The Meadows School (Las Vegas, NV) | 44 | Sage Ridge School (Reno, NV) | DNP |
| West I | Bye | 0 | Liberty Common High School (Fort Collins, CO) | 55 |
| West II | Bye | 0 | Anderson HS (Austin, TX) | DNP |
| West II | The Classical Academy High School (Colorado Springs, CO) | 43 | Northside Health Careers HS (San Antonio, TX) | 66 |
| West II | Thomas Jefferson High School Gold (Denver, CO) | 29 | Thomas Jefferson High School Purple (Denver, CO) | DNP |