DNP = did not play in this round
| Region | Team 1 | Team 2 | ||
|---|---|---|---|---|
| Atlantic I | Gonzaga College High School (Washington, DC) | 35 | Hayfield Secondary School (Alexandria, VA) | 25 |
| Atlantic I | Flint Hill School (Oakton, VA) | 45 | Thomas A Edison High School (Alexandria, VA) | 34 |
| Atlantic I | Oakton High School (Vienna, VA) | 35 | Thomas Jefferson HS for Science and Technology (Alexandria, VA) | 69 |
| Atlantic II | Franklin Academy High School Purple (Wake Forest, NC) | 31 | Covenant Day School Purple (Matthews, NC) | DNP |
| Atlantic II | Covenant Day School Gold (Matthews, NC) | 37 | Franklin Academy High School Gold (Wake Forest, NC) | 29 |
| Atlantic II | Thales Academy Rolesville (Rolesville, NC) | DNP | Plant High School (Tampa, FL) | 49 |
| Central I | St. Mark's School of Texas (Dallas, TX) | 46 | Amarillo High School Gold (Amarillo, TX) | 36 |
| Central I | Amarillo High School Purple (Amarillo, TX) | DNP | Fort Worth Country Day (Fort Worth, TX) | 35 |
| Central I | Providence Preparatory School (Belton, TX) | DNP | Trinity Valley School (Fort Worth, TX) | 49 |
| Central II | Clements High School Gold (Sugar Land, TX) | 26 | Clements High School Purple (Sugar Land, TX) | 44 |
| Central II | Klein Collins High School (Klein, TX) | 42 | Aristoi Classical Academy (Katy, TX) | 33 |
| Central II | Highlands Latin School Houston Gold (Houston, TX) | DNP | Highlands Latin School Houston Purple (Houston, TX) | DNP |
| Midwest I | University School of Milwaukee (River Hills, WI) | DNP | Brookfield Academy (Brookfield, WI) | 50 |
| Midwest I | Brookfield East High School (Brookfield, WI) | 49 | Collinsville High School (Collinsville, IL) | 34 |
| Midwest I | Parnassus Preparatory School (Maple Grove, MN) | 43 | Saint Louis Priory School (St. Louis, MO) | 38 |
| Midwest II | St. Xavier High School (Cincinnati, OH) | DNP | Homestead High School (Fort Wayne, IN) | DNP |
| Midwest II | North Central High School (Indianapolis, IN) | 29 | St. Edward High School (Lakewood, OH) | DNP |
| Midwest II | Covington Classical Academy (Covington, KY) | 46 | Northwest Ohio Classical Academy (Toledo, OH) | 33 |
| Northeast I | Ridgewood High School (Ridgewood, NJ) | 35 | Stuyvesant High School Gold (New York, NY) | 49 |
| Northeast I | Stuyvesant High School Purple (New York, NY) | 45 | Garden City High School (Garden City, NY) | 41 |
| Northeast I | Hampden Academy Purple (Hampden, ME) | 37 | Hampden Academy Gold (Hampden, ME) | 33 |
| Northeast II | Boys' Latin of Philadelphia Charter High School (Philadelphia, PA) | 57 | Lower Merion High School (Ardmore, PA) | 43 |
| Northeast II | Harriton HS Purple (Bryn Mawr, PA) | 62 | Harriton HS Gold (Bryn Mawr, PA) | 45 |
| Northeast II | IvyMind Academy (Garnet Valley, PA) | 91 | Owen J. Roberts High School (Pottstown, PA) | 44 |
| Northeast III | State College Area High School Gold (State College, PA) | 30 | Beaumont School (Cleveland Heights, OH) | 32 |
| Northeast III | Winchester High School (Winchester, MA) | 36 | State College Area High School Purple (State College, PA) | 55 |
| Northeast III | The Linsly School (Wheeling,, WV) | 30 | Bexley Middle & High School (Columbus, OH) | DNP |
| Pacific I | 'Iolani School Gold (Honolulu, HI) | 52 | Sage Hill School (Newport Coast, CA) | 62 |
| Pacific I | Pacifica Christian HS Santa Monica (Santa Monica, CA) | 39 | 'Iolani School Purple (Honolulu, HI) | 32 |
| Pacific I | University High School Gold (Irvine, CA) | 48 | University High School Purple (Irvine, CA) | 51 |
| Pacific II | Basis Ahwatukee Purple (Phoenix, AZ) | 42 | Brophy College Preparatory (Phoenix, AZ) | 47 |
| Pacific II | Trivium Preparatory Academy (Goodyear, AZ) | 45 | Phoenix Country Day School Purple (Paradise Vly, AZ) | 66 |
| Pacific II | Phoenix Country Day School Gold (Paradise Vly, AZ) | 47 | Basis Ahwatukee Gold (Phoenix, AZ) | 68 |
| South I | George Walton Academy Gold (Monroe, GA) | DNP | Walton High School Purple (Marietta, GA) | 56 |
| South I | Milton High School (MIlton, GA) | 44 | DeKalb School of the Arts (Avondale Estates, GA) | DNP |
| South I | Walton High School Gold (Marietta, GA) | 52 | George Walton Academy Purple (Monroe, GA) | DNP |
| South II | Maclay School (Tallahassee, FL) | DNP | Society of Themistocles (Oviedo, FL) | DNP |
| South II | Thomas County Schools (Thomasville, GA) | 34 | Alabama School of Fine Arts Gold (Birmingham, AL) | DNP |
| South II | Alabama School of Fine Arts Purple (Birmingham, AL) | DNP | Oak Hall School (Gainesville, FL) | 31 |
| South III | Dreher HS (olumbia, SC) | DNP | Central Magnet School (Murfreesboro, TN) | DNP |
| South III | Memphis University School Purple (Memphis, TN) | 50 | Clarksville High School (Clarksville, TN) | 38 |
| South III | Summit Country Day School (Cincinnati, OH) | DNP | Memphis University School Gold (Memphis, TN) | 47 |
| West I | CHAO (Omaha, NE) | DNP | Sage Ridge School (Reno, NV) | DNP |
| West I | The Harker School (San Jose, CA) | 63 | Bye | 0 |
| West I | The Meadows School (Las Vegas, NV) | 44 | Liberty Common High School (Fort Collins, CO) | 43 |
| West II | Bye | 0 | Northside Health Careers HS (San Antonio, TX) | DNP |
| West II | Anderson HS (Austin, TX) | 37 | Thomas Jefferson High School Gold (Denver, CO) | DNP |
| West II | The Classical Academy High School (Colorado Springs, CO) | DNP | Thomas Jefferson High School Purple (Denver, CO) | DNP |