DNP = did not play in this round; DQ = disqualified in this round
| Region | Team 1 | Team 2 | ||
|---|---|---|---|---|
| Atlantic I | Thomas A Edison High School (Alexandria, VA) | DNP | Mcdonogh School (Owings Mills, MD) | 32 |
| Atlantic I | Thomas Jefferson HS for Science and Technology (Alexandria, VA) | 53 | Flint Hill School (Oakton, VA) | DNP |
| Atlantic I | Gonzaga College High School (Washington, DC) | 39 | Trinity Upper School (Durham, NC) | 42 |
| Atlantic II | Ridgewood High School (Ridgewood, NJ) | 40 | Covenant Day School Purple (Matthews, NC) | DNP |
| Atlantic II | Thomas Jefferson Classical Academy (Mooresboro, NC) | 36 | Owen J. Roberts High School Gold (Pottstown, PA) | 41 |
| Atlantic II | Owen J. Roberts High School Purple (Pottstown, PA) | 26 | Covenant Day School Gold (Matthews, NC) | 25 |
| Atlantic III | Lower Merion High School (Ardmore, PA) | 32 | Boys' Latin of Philadelphia Charter High School (Philadelphia, PA) | 25 |
| Atlantic III | Radnor High School Purple (Radnor, PA) | DNP | Harriton HS Gold (Bryn Mawr, PA) | 49 |
| Atlantic III | Radnor High School Gold (Radnor, PA) | DNP | Harriton HS Purple (Bryn Mawr, PA) | 51 |
| Central I | Trinity Valley School (Fort Worth, TX) | 28 | St. Mark's School of Texas Purple (Dallas, TX) | DNP |
| Central I | All Saints' Episcopal School (Fort Worth, TX) | 36 | Bye | 0 |
| Central I | St. Mark's School of Texas Gold (Dallas, TX) | DNP | Fort Worth Country Day (Fort Worth, TX) | 37 |
| Central II | Klein Collins High School (Klein, TX) | 31 | Clements High School Purple (Sugar Land, TX) | 32 |
| Central II | Clements High School Gold (Sugar Land, TX) | 28 | St. John's School (Houston, TX) | 36 |
| Central II | St. Thomas High School (Houston, TX) | DNP | Aristoi Classical Academy (Katy, TX) | 35 |
| Central III | Northside Health Careers HS (San Antonio, TX) | 36 | St. Mary's Hall (San Antonio, TX) | DNP |
| Central III | Westlake High School Gold (Austin, TX) | 37 | Westlake High School Purple (Austin, TX) | 28 |
| Central III | Anderson HS (Austin, TX) | 38 | Austin High School (Austin, TX) | DNP |
| Central IV | Amarillo High School Purple (Amarillo, TX) | 30 | Amarillo High School Gold (Amarillo, TX) | 31 |
| Central IV | The Classical Academy High School Gold (Colorado Springs, CO) | 29 | IvyMind Academy Gold (Online - USA) | DNP |
| Central IV | IvyMind Academy Purple (Online - USA) | 76 | The Classical Academy High School Purple (Colorado Springs, CO) | 38 |
| Midwest I | Beaumont School (Cleveland Heights, OH) | 22 | Brookfield Academy Gold (Brookfield, WI) | 23 |
| Midwest I | Divine Savior Holy Angels HS (Milwaukee, WI) | DNP | Brookfield East High School (Brookfield, WI) | 49 |
| Midwest I | Brookfield Academy Purple (Brookfield, WI) | 65 | University School of Milwaukee (River Hills, WI) | DNP |
| Midwest II | Parnassus Preparatory School (Maple Grove, MN) | 27 | Crown Point High School (Crown Point, IN) | 36 |
| Midwest II | Catholic Memorial High School Gold (Waukesha, WI) | 39 | Collinsville High School (Collinsville, IL) | 35 |
| Midwest II | Saint Louis Priory School (St. Louis, MO) | 28 | Catholic Memorial High School Purple (Waukesha, WI) | 30 |
| Midwest III | North Central High School (Indianapolis, IN) | 21 | Covington Classical Academy (Covington, KY) | 45 |
| Midwest III | Clarksville High School (Clarksville, TN) | 40 | Homestead High School (Fort Wayne, IN) | 42 |
| Midwest III | Sycamore Community Schools (Cincinnati, OH) | DNP | Louisville Classical Academy (Louisville, KY) | DNP |
| Northeast I | Hampden Academy Gold (Hampden, ME) | 33 | John Bapst Memorial High School (Bangor, ME) | 25 |
| Northeast I | Fryeburg Academy (Fryeburg, ME) | 36 | Foxcroft Academy Purple (Dover-Foxcroft, ME) | DNP |
| Northeast I | Foxcroft Academy Gold (Dover-Foxcroft, ME) | DNP | Hampden Academy Purple (Hampden, ME) | 36 |
| Northeast II | Winchester High School (Winchester, MA) | 33 | Roxbury Latin (West Roxbury, MA) | 62 |
| Northeast II | Goffstown High School (Goffstown, NH) | DNP | Shrewsbury High School (Shrewsbury, MA) | 38 |
| Northeast II | Phillips Exeter Academy Gold (Exeter, NH) | 54 | Phillips Exeter Academy Purple (Exeter, NH) | 59 |
| Pacific I | App-Ark Institute Purple (Online - China) | DNP | Thomas Jefferson High School (Denver, CO) | DNP |
| Pacific I | 'Iolani School Gold (Honolulu, HI) | 41 | The Harker School (San Jose, CA) | 50 |
| Pacific I | 'Iolani School Purple (Honolulu, HI) | 50 | App-Ark Institute Gold (Online - China) | DNP |
| Pacific II | Woodbridge High School (Irvine, CA) | 31 | Sage Hill School (Newport Coast, CA) | 37 |
| Pacific II | The Meadows School (Las Vegas, NV) | 42 | University High School Purple (Irvine, CA) | 40 |
| Pacific II | Pacifica Christian HS Santa Monica (Santa Monica, CA) | 25 | University High School Gold (Irvine, CA) | 44 |
| Southeast I | Berkeley Preparatory School (Tampa, FL) | 48 | Oak Hall School (Gainesville, FL) | 52 |
| Southeast I | The King’s Academy (West Palm Bch, FL) | 5 | Maclay School Gold (Tallahassee, FL) | DNP |
| Southeast I | Plant High School (Tampa, FL) | 24 | Maclay School Purple (Tallahassee, FL) | DNP |
| Southeast II | Walton High School Purple (Marietta, GA) | 44 | Houston High Gold (Germantown, TN) | DNP |
| Southeast II | Walton High School Gold (Marietta, GA) | 45 | Houston High Purple (Germantown, TN) | DNP |
| Southeast II | Milton High School (MIlton, GA) | 48 | Wheeler High School (Marietta, GA) | DNP |
| Southwest | BASIS Phoenix (Phoenix, AZ) | 29 | Brophy College Preparatory (Phoenix, AZ) | DNP |
| Southwest | Phoenix Country Day School Gold (Paradise Vly, AZ) | 47 | Phoenix Country Day School Purple (Paradise Vly, AZ) | 45 |
| Southwest | Basis Ahwatukee (Phoenix, AZ) | 36 | Basis Ahwatukee/Phoenix (Phoenix, AZ) | 42 |