DNP = did not play in this round
| Region | Team 1 | Team 2 | ||
|---|---|---|---|---|
| Atlantic I | Thomas Jefferson HS for Science and Technology (Alexandria, VA) | 57 | Gonzaga College High School (Washington, DC) | 41 |
| Atlantic I | Mcdonogh School (Owings Mills, MD) | DNP | Trinity Upper School (Durham, NC) | 38 |
| Atlantic I | Flint Hill School (Oakton, VA) | DNP | Thomas A Edison High School (Alexandria, VA) | 30 |
| Atlantic II | Thomas Jefferson Classical Academy (Mooresboro, NC) | 41 | Owen J. Roberts High School Purple (Pottstown, PA) | 27 |
| Atlantic II | Covenant Day School Purple (Matthews, NC) | DNP | Covenant Day School Gold (Matthews, NC) | 35 |
| Atlantic II | Owen J. Roberts High School Gold (Pottstown, PA) | 39 | Ridgewood High School (Ridgewood, NJ) | 46 |
| Atlantic III | Radnor High School Purple (Radnor, PA) | 34 | Radnor High School Gold (Radnor, PA) | DNP |
| Atlantic III | Boys' Latin of Philadelphia Charter High School (Philadelphia, PA) | 23 | Harriton HS Purple (Bryn Mawr, PA) | 50 |
| Atlantic III | Harriton HS Gold (Bryn Mawr, PA) | 58 | Lower Merion High School (Ardmore, PA) | 35 |
| Central I | All Saints' Episcopal School (Fort Worth, TX) | 34 | St. Mark's School of Texas Gold (Dallas, TX) | 48 |
| Central I | St. Mark's School of Texas Purple (Dallas, TX) | 51 | Fort Worth Country Day (Fort Worth, TX) | 36 |
| Central I | Bye | 0 | Trinity Valley School (Fort Worth, TX) | 42 |
| Central II | Clements High School Gold (Sugar Land, TX) | 31 | St. Thomas High School (Houston, TX) | DNP |
| Central II | Clements High School Purple (Sugar Land, TX) | 30 | Aristoi Classical Academy (Katy, TX) | 34 |
| Central II | St. John's School (Houston, TX) | 28 | Klein Collins High School (Klein, TX) | DNP |
| Central III | Westlake High School Gold (Austin, TX) | 31 | Anderson HS (Austin, TX) | 46 |
| Central III | St. Mary's Hall (San Antonio, TX) | DNP | Austin High School (Austin, TX) | DNP |
| Central III | Westlake High School Purple (Austin, TX) | 35 | Northside Health Careers HS (San Antonio, TX) | 49 |
| Central IV | The Classical Academy High School Gold (Colorado Springs, CO) | 40 | IvyMind Academy Purple (Online - USA) | 71 |
| Central IV | Amarillo High School Gold (Amarillo, TX) | 21 | The Classical Academy High School Purple (Colorado Springs, CO) | 33 |
| Central IV | IvyMind Academy Gold (Online - USA) | DNP | Amarillo High School Purple (Amarillo, TX) | 19 |
| Midwest I | Divine Savior Holy Angels HS (Milwaukee, WI) | 33 | Brookfield Academy Purple (Brookfield, WI) | 68 |
| Midwest I | Brookfield Academy Gold (Brookfield, WI) | 32 | University School of Milwaukee (River Hills, WI) | DNP |
| Midwest I | Brookfield East High School (Brookfield, WI) | 55 | Beaumont School (Cleveland Heights, OH) | 32 |
| Midwest II | Catholic Memorial High School Gold (Waukesha, WI) | 25 | Saint Louis Priory School (St. Louis, MO) | 30 |
| Midwest II | Crown Point High School (Crown Point, IN) | 35 | Catholic Memorial High School Purple (Waukesha, WI) | 30 |
| Midwest II | Collinsville High School (Collinsville, IL) | 31 | Parnassus Preparatory School (Maple Grove, MN) | 33 |
| Midwest III | Clarksville High School (Clarksville, TN) | 45 | Sycamore Community Schools (Cincinnati, OH) | 49 |
| Midwest III | Covington Classical Academy (Covington, KY) | 48 | Louisville Classical Academy (Louisville, KY) | 41 |
| Midwest III | Homestead High School (Fort Wayne, IN) | 38 | North Central High School (Indianapolis, IN) | 36 |
| Northeast I | Fryeburg Academy (Fryeburg, ME) | 33 | Foxcroft Academy Gold (Dover-Foxcroft, ME) | DNP |
| Northeast I | John Bapst Memorial High School (Bangor, ME) | 39 | Hampden Academy Purple (Hampden, ME) | 45 |
| Northeast I | Foxcroft Academy Purple (Dover-Foxcroft, ME) | DNP | Hampden Academy Gold (Hampden, ME) | 33 |
| Northeast II | Goffstown High School (Goffstown, NH) | 34 | Phillips Exeter Academy Gold (Exeter, NH) | 65 |
| Northeast II | Roxbury Latin (West Roxbury, MA) | 75 | Phillips Exeter Academy Purple (Exeter, NH) | 64 |
| Northeast II | Shrewsbury High School (Shrewsbury, MA) | 27 | Winchester High School (Winchester, MA) | 33 |
| Pacific I | 'Iolani School Gold (Honolulu, HI) | 45 | 'Iolani School Purple (Honolulu, HI) | 48 |
| Pacific I | Thomas Jefferson High School (Denver, CO) | DNP | App-Ark Institute Gold (Online - China) | DNP |
| Pacific I | The Harker School (San Jose, CA) | 69 | App-Ark Institute Purple (Online - China) | DNP |
| Pacific II | The Meadows School (Las Vegas, NV) | 34 | Pacifica Christian HS Santa Monica (Santa Monica, CA) | 31 |
| Pacific II | Sage Hill School (Newport Coast, CA) | 39 | University High School Gold (Irvine, CA) | 43 |
| Pacific II | University High School Purple (Irvine, CA) | 27 | Woodbridge High School (Irvine, CA) | 39 |
| Southeast I | The King’s Academy (West Palm Bch, FL) | 26 | Plant High School (Tampa, FL) | 24 |
| Southeast I | Oak Hall School (Gainesville, FL) | 54 | Maclay School Purple (Tallahassee, FL) | DNP |
| Southeast I | Maclay School Gold (Tallahassee, FL) | DNP | Berkeley Preparatory School (Tampa, FL) | 45 |
| Southeast II | Walton High School Gold (Marietta, GA) | 49 | Milton High School (MIlton, GA) | 30 |
| Southeast II | Houston High Gold (Germantown, TN) | 36 | Wheeler High School (Marietta, GA) | DNP |
| Southeast II | Houston High Purple (Germantown, TN) | 36 | Walton High School Purple (Marietta, GA) | 49 |
| Southwest | Phoenix Country Day School Gold (Paradise Vly, AZ) | 41 | Basis Ahwatukee (Phoenix, AZ) | 38 |
| Southwest | Brophy College Preparatory (Phoenix, AZ) | DNP | Basis Ahwatukee/Phoenix (Phoenix, AZ) | 47 |
| Southwest | Phoenix Country Day School Purple (Paradise Vly, AZ) | 51 | BASIS Phoenix (Phoenix, AZ) | 26 |